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SUMO is a ubiquitin-like protein that is conjugated to proteins via a ubiquitin-like cascade, involving an E1-like activating enzyme, an E2-conjugating enzyme, and an E3 ligase. SUMO conjugation has recently been shown to be involved in several plant processes, but not in artichoke (Cynara scolymus L.) cold stress response. In the present study, we focused on the effect of ambient temperature on the endogenous SUMOylation pathway in artichoke. Cold stress at 4 degrees C induced a general decrease in the expression of the genes involved in artichoke SUMOylation at 4 degrees C as compared to the control at 25 degrees C. Accumulation of the SUMO-1 protein was also lower in artichoke leaves and stems exposed to cold stress at 4 degrees C. High levels of the SUMO-1 protein were detected in the green leaves and roots under cold stress at 25 degrees C. When artichoke plants were kept at 4 degrees C for 24 h, an induction of the endogenous SUMOylation pathway was observed in artichoke leaves, stems, and roots, as evidenced by increased levels of the SUMO-1 protein and higher transcript accumulation of the genes involved in artichoke SUMOylation. Moreover, increased accumulation of the SUMO-1 protein was observed in the green leaves, stems, and roots under cold stress at 25 degrees C. These results suggest that the endogenous SUMOylation pathway is activated in artichoke, leading to increased levels of the SUMO-1 protein. TXT2HTML Converter Crack With Serial Key Free [Mac/Win] Convert TXT file to HTML document! TXT2HTML Converter is a lightweight application designed to convert plain text format into HTML webpages after customizing some basic settings. It doesn't require previous HTML experience and doesn't need installation. 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Evaluation and conclusion The utility is clearly aimed at casual users, since it doesn't contain more advanced options. The fact that it can read only TXT file means that it cannot process tables, images, or other graphical elements. Unsurprisingly, it uses a low amount of CPU and RAM, so it doesn't hog system resources. Although it hasn't been updated for a long time, it still works on newer Windows editions and doesn't require administrative privileges. To conclude, TXT2HTML Converter is mostly useful for those who want to learn how to code in HTML by examining the source code of the generated webpages. However, due to its lack of advanced options, it cannot be used for more serious purposes, such as creating websites. 2: Portable TXT2HTML Converter TXT2HTML Converter is a portable text-to-html converter, which will save your time, in order to make the full use of your language skills. This is... 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